SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. com. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. SpectraMax Spectrofluorometers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Multimode Reader Comparison Chart. Recertification is recommended every two years. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Detection of pro-inflammatory factorsThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Reader Documentation Version No. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. For prep samples treated with the ELISA kit reagents, it is fast and user friendly. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Brand New. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 0112-0115 F . use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. 7°C and the bottom to 36. 40266 Email: drugdiscoverytech@lifetech. 9000-0756. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. ". SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. In an analogous manner, the same sample names were assigned to a CuvetteSet section. 313 reference gain check fail. Molecular devices SpectraMax M5 Pdf User Manuals. 00800 665 32860. 2. PURPOSE. SpectraMax iD5 Multi-Mode Microplate Reader. Information on how to. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Published Dec 16, 2020 | Updated Dec 16, 2020. This process should take about 3 - 5 minutes. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. 000000 out of 5. Tecan Infinite M1000 Microplate Reader w/ Injectors $10,000. SpectraMax M5 Multi-Mode Microplate Reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. There is currently no charge for using this plate reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax® M5 Multi-Mode Microplate Re. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). 0 . Unplug the power cable from the back of the instrument. Five preferred detection modes. Page 1 of 8 . spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Figure 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. Reader Documentation Version No. 7°C (gradient heating design). Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 3: The cuvette chamber. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Gemini EM Reader Top and Bottom OpticsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. PERKIN ELMER Wallac Victor 2V Microplate Reader. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Winnersh Triangle. Plate reader instructions Computer. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 2. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 87. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with abs. We therefore gathered salient. SpectraMax i3 reader settings for IMAP PDE assays. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The following measurements are automatically made by SoftMax Pro Software: baseline noise, dark noise, optical alignment, wavelength accuracy, photometric linearity and wavelength precision. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Choose cables that meet the requirement described in this appendix. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Then the plate was returned to the. Thank you!. 0 out of 5 $ 16,499. SpectraMax M5 microplate reader. Our absorbance plate readers feature our PathCheck Sensor technology and. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 0 out of 5 $ 17,999 $ 16,995. 12. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Compatible Fluorescence Polarization Plate Readers. Specifications Plate mixing modes Selectable: off, once prior to any reading, and once prior to and between kinetic readings Plate mixing duration Selectable: 0 to 999 seconds (three-second default. Molecular Devices microplate readers. The guide also covers the software features, the specifications, and the accessories of the systems. SeePlateAdapteronpage20. The SpectraMax M5 is a multi-detection, monochromator-based reader with both topand bottom-read capability. B. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. 1. Example: [Drawer]. 1. Align the two slots on the bottle holder with the two pegs on the rail. The plate is set up for reading and images are acquired according to specified parameters. Multimode Readers Comparison Chart. SoftMax Pro. 1. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. , contact. The following document is intended. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Figure 4: Transcreener ADP. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 0 out of 5 $ 15,999. 0 . book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Using the. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. Spectramax M5. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. edu, 814-863-3503. SpectraMax® microplate readers: reader check and error codes Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader,. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Description 1. Similar settings will work for other SpectraMax readers with fluorescence detection. Category 2 and 3 microplate readers use gradient heating as well. 8. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. es •. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. The BD plate dimensions are 5. Choose from a three- (M3), four- (M4), orProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The computer controlling the plate reader runs Windows XP. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. These robust readers have been placed in labs from Antarctica to. These lab workhorses are used in drug discovery. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. There is currently no charge for using this plate reader. 40266 Email: [email protected] OD ± 3. 2. Read More. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The multi-mode. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. After the plate is read, data will appear in the plate section: 10. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 3: The cuvette chamber. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 FlexStation® 3 Multi-Mode Microplate Reader. The Analyst GT is equipped with 20 plate magazines in a bi-directional stacker. Information on how to reserve the plate readerThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. For more sample throughput, the system canThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Italic and boldface type are used for emphasis. 000 to 3. For more information please visit us at: M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. 9000-0756. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. There is currently no charge for using this plate reader. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 0 . Choose from a three- (M3), four-. D 49 7. Standard curves were plotted using SoftMax® Pro Software (Figure 1). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. . 2. SpectraMax M2, M2e;. 12. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The drawing shows a standard microplate of 15 mm height covered with a lid. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Figure 1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Instructions. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. Published Dec 16, 2020 | Updated Dec 16, 2020. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. Compact and tunable absorbance microplate readers DATA SHEET. View online or download Molecular devices SpectraMax M5e User Manual. 005) Photometric PrecisionThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. By offering two new configurations of multi-mode readers that deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an even greater combination of detection, application, and budgetary needs. 1. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. +44 118 944 8000. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 0 . Here the delta mP was 336, and Z’ factor was 0. Maximum signal with lowest background. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Microplate Reader Shelf. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Wide range of applications. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. IMAP assays are based on binding of. l. With optimized reagents, option to operate the readers. Supported Plates and Cuvettes. Trends in Immunology. Molecular Device SpectraMax M2 Plate Reader - Certified with Warranty. The chamber. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax M5 e reader has the same performance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF. Catalog Number: M5. Data analysis Standard curves were plotted using the quadratic curve fit in SoftMax Pro Software, and the concentrations of cell lysates were calculated from the standard curves by the software. 2. Features. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integratedWhat types of plates can be used with StakMax? SBS Standard 96 and 384 well plates with plate height of 12-16 mm are recommended. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Spectramax Softmax Pro 6. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. com 10. 8. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. For a higher . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. D 1. Please talk to your your local Tecan representative for the right configuration which suits your application demands. The SpectraMax M2 and M2e are versatile and reliable. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Ensure data accuracy of your reader. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. The dual monochromator optics allow. These robust readers have been placed in labs from. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. 3) At the bottom of the strip, click on "Show. SpectraMax® M5/M5e Microplate Reader Have A Question? Contact Our Technical Support Team NA: 800-955-6288 Or INTL: 760-603-7200 Ext. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. Fluorescence Microplate Reader Comparison Chart. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). Standard features include a cuvette port, spectral scanning in 1 nm increments, and. 4. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The plate is set up for reading and images are acquired according to specified parameters. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. No accessories are needed and SoftMax® Pro Software. Third this recommendation. 0200-0729. 100 µL of AK Reagent Working Solution was added to each well, and the plate was read within 30 minutes. Be sure to insert . Particles in the light beam at the time of aProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Molecular Devices SpectraMax M2 Multimode Plate Reader. the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. SpectraMax i3x Multi-Mode Microplate Re. Wait 20 seconds. The calculated LOQ values were 0. 99. eu@moldev. SpectraMax M5 Multi-Mode Microplate Reader. View online or download Molecular devices SpectraMax M5 User Manual. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. 2. 4. Human macrophages choreograph tissue development. 200 ‚ 1000 nm. The M5 can read volumes down to 2uL with the. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Prepare your i3/i3x instrument for shipment. Description. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . . The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. After the plate is read, data will appear in the plate section: 11. 1. The chamber. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The plate reader. Test up to 3. 2. 0 out of 5 $ 5,999. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The dual monochromator optics allow the. 12. e. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. edu) to be added as a trained user in iLab. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. book Page 21 Wednesday, July 7, 2010 2:09 PM. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. TR-FRET assay. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. 0% ± 0. When a feature or capability applies to only certain readers, this exception is noted. Most papers they cultured bacteria to OD600>0. Description The Onboard Microprocessor. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Following treatment the samples were assayed for protease activity in triplicate using the Pierce Fluorescent Protease Assay Kit (Thermo Scientific, Waltham USA),. Your applications, your modes, your choice. 40266The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The Infinite and Spark multimode microplate readers are modular and upgradeable. Molecular Devices SpectraMax Plus 384. Gemini XPS. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. 00. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Our lab regularly left plates with aqueous buffer in them in the plate reader (service contract lapsed for several years. BENEFITS • No filters needed • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 2. 1) Select the correct plate and the wells that you want to measure. 00 USD. The M5e Reader is easily automated. The dedicated luminescence PMT has extremely low background counts and superior performance in the blue wavelengths. Bottom-reading is usually recommended for cell-based assay with adherent cells. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. both cuvette-based and microplate reader applications SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. A dialog box will appear, requesting you to confirm that the read is a pre-read. Microplate Readers. Please contact Tatiana to reserve the plate reader time: tnl1@psu. 1. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Copia stocks a full line of used microplate readers. Uncontrolled Copy for Training and Reference Purposes Only . Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9660-665 Eskdale Road. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. 03 in x 3. 9. For SpectraMax L or LMax II, LMax II. 8. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSetup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SeePlateAdapteronpage20. 1. Comes from Lab closure Approx. N.